Protein transport from ER to golgi to lysosome

Published by Darron Toy on

hello friends welcome back to another video tutorial from somos analogy in this video lecture we want to talk about the transport of proteins into the lysosome till now we've been talking about the transport of proteins in different organelles of a cell we've seen the transporter proteins in mitochondria transport of the proteins to the chloroplast if the nucleus and paroxysms but all those pathways that we saw like transport of a protein to the mitochondria or chloroplasts they requires the transport of the protein from cytosol itself because in those case the proteins are made in the cytosol without being involved and ended up in in the inside the lumen of endoplasmic reticulum but in this case of transferring a protein into the lysosome we will see that the protein is produced and inserted into inside the endoplasmic reticulum then it will be packaged inside the vesicle transported from there and then only they will form lysosomes it's a part of a endomembrane system how it looks like if you think of an idea that this are the endoplasmic reticulum somehow linked let's imagine should try lake let's say let's say this is an endoplasmic reticulum and right after that we have the Golgi apparatus and so on we have the Golgi apparatus and let's say this is all lysosome so to understand the pathway of how a protein is delivered inside the lysosome you need to know the sequence of the events and to understand the sequence of the evils it starts with endoplasmic reticulum then the Golgi apparatus then the other structures like in those room any other structures and then finally with the destination called lysosome so what is it we'll begin here the idea of protein synthesis is going on in the endoplasmic reticulum it occurs because the ribosome is there in the endoplasmic reticulum and then it is making the polypeptide sequence making the polypeptide sequences and once the polypeptide sequence is made where the protein is made it will be inserted inside the endoplasmic reticulum lumen so once the protein is inserted inside the endoplasmic reticulum lumen then the second job is to make a vesicle where the protein will be inserted so vesicle start forming including the target proteins and then they will be delivered towards the Golgi apparatus so if I draw it here the vesicle start forming the structure like that and here will be this proteins moving inside the physical and that protein will now go and fuse with the Golgi apparatus actually those vesicles will fuse with themselves as a result the Golgi would start actin ecology is formed with the help of the interaction of those vesicles and the target protein is transferred to the Golgi apparatus so once that thing is done then what happens in golgi is those proteins are modified in different sense there are different chemical modifications in this case let's say the model let's say this protein is the hydraulic routing that we are tracking now the protein that is in the Golgi apparatus should be modified in this case the modification is let's say a addition of sugar very same man owns edition of manners and not only the addition of manners but also there is another medic modification that is the modification of manuals itself that is phosphorylation of Manos so the manual gets phosphorylated and converting the six phosphate so mannose 6-phosphate is formed in the structure of the hydrolase enzyme and that all alteration takes place in the Golgi apparatus during the movement of the protein through the Golgi channels or Golgi Network now what mannose 6-phosphate is formed then finally they are same protein in towards the terminal part of the Golgi because in if you look at the Golgi there are since medial entrance so once it's there at the end those Manos along with the those those proteins hydrolyze with the mannose 6-phosphate binds to the mannose-6-phosphate receptor that is also present in the Golgi apparatus membrane embedded in the membrane so if you look at here let's let's draw the mannose 6-phosphate except with a different color let's do it with the red color so this is the vanos 6-phosphate receptor which binds to the mannose-6-phosphate of the hydrolase enzyme and once this thing is done then it will start creating the vesicles and start inching out from the Golgi Network and then it is transported if I draw it through the structures then it will be transported towards lysosome and even before becoming a lysosome this vesicle cell itself have the capability to convert itself into a lysosome the only difference is like contains many of these enzymes – and many of the 50 days lipase and many of these enzymes all together and also they have a very acidic pH so at the very beginning if you look at the if you look at the physical it has those mannose 6-phosphate receptor while the hydrolase with mannose 6-phosphate attaches to it at this particular condition there is a ATPase pump or akansha proton pump that is present through this pump ATP hydrolysis is required as an energy source there and that helps driving protons inside this physical structure which is known as early endosome this is start an initiation point of formation of a lysosome known as early in the room the into the endosome and mattified releases there and it helps in the transport of protons inside the vesicle that makes the environment acidic due to this acidic environment that 6-phosphate of this mannose residue gets released and as a result the target hydrolase is also getting a relief so as the as the acidity as their acidity slowly starts to rise there the protein target protein along with the mannose residue it's released because of phosphate gets released due to the high acidity and it also caused this other structure of the of the vesicle that is -6 receptor 2 completely dissociated from the hydronium side and as a result we have this high proton contained inside now and we also have a one hydrologic design inside this is the way many different hydrolytic enzymes are delivered inside this matured Enzo and also my quads call it like late endosomes now once this latencies in do some slowly start to affirm more and more of this hydrolyzing enzyme lipase protease and all this type of enzyme slowly start to build up and making this acidic environment then that sac will be known as light ism so why are we talking about delivery of proteins to the lysosome it's not like lysosome is preformed and the protein gets delivered but is actually during the process of this transport lysosome is being formed from early endosome they let in the room then the modification of that into the lysosome that is the process of lysosomal protein transport I hope you understand this video if you like this video please hit the like button share this video with your friends and subscribe to my channel to get more and more videos like that thank you


ETERNALR RAFJAV · May 15, 2019 at 1:18 pm

I dnt understand how can a person dislike these free and useful lectures..the people who dislike must be envious to you..

kamal sutradhar · May 15, 2019 at 1:18 pm

Sir I'm a student of bsc in botany ….and I can't understand the topics ….but your videos help me very much …thank you sir for your valuable videos

M.Toib · May 15, 2019 at 1:18 pm


Rahit Biswas · May 15, 2019 at 1:18 pm


Gavin Kisebach · May 15, 2019 at 1:18 pm

Excellent explanation and great marker skills; you have my sub, sir.

krishna barge · May 15, 2019 at 1:18 pm

Ur pronunciation is very very clear sir

Sahila Syed · May 15, 2019 at 1:18 pm

Thank u sir…great lecture๐Ÿ‘

Subha Jani · May 15, 2019 at 1:18 pm

Thank You Shomu Sir… Ur lessons are easy to understand.. Do post more and more lessons and one more thing sir if u can provide notes or reference it would be an immense help… โ˜บ๏ธโ˜บ๏ธโ˜บ๏ธ

Joe_ · May 15, 2019 at 1:18 pm

I learn biochemistry in turkish language I cant understand lectures because of language but I completed most of the topics by using your videos. You are so helpful may God bless you

Subhojit Bag · May 15, 2019 at 1:18 pm

It's Helpful

Varsha Varsha Gaur · May 15, 2019 at 1:18 pm

Thnku so much sir

Vekuzo Chizo · May 15, 2019 at 1:18 pm

Thank you sir

Sally Zee · May 15, 2019 at 1:18 pm

Can you please explain anterograde and retrograde difference in pathway

Ranjan Jena · May 15, 2019 at 1:18 pm

Hi I am Sai and I have a question that where are lysosome synthesized?

shaninee yadav · May 15, 2019 at 1:18 pm

thanks sir u r the best teacher for the prepration of csir

TRISHA MONDAL · May 15, 2019 at 1:18 pm

Thank u so much. Without your teaching i can't pass my internal exam.

Sneha Mital · May 15, 2019 at 1:18 pm

When the phosphate group is released and the late endosome is formed… where did the mannose sugar go?

Tahir Tantary · May 15, 2019 at 1:18 pm


Asinjit Das · May 15, 2019 at 1:18 pm


Umar Farooq · May 15, 2019 at 1:18 pm

very useful for my sister's examinations

SHREYA BHARTI · May 15, 2019 at 1:18 pm

Is hydrolase is protein or enzyme

Trini Gyal · May 15, 2019 at 1:18 pm

Thank you!!!

Leonida Monaco · May 15, 2019 at 1:18 pm

Very helpful to understand the simple mechanism.. To have a great picture. Thanks

faiz abbas · May 15, 2019 at 1:18 pm

thank you so much

Ananya buri · May 15, 2019 at 1:18 pm

Thank u so much sir for your helpful video, you make this topic so easy to understand๐Ÿ˜„

Pushpa Lata · May 15, 2019 at 1:18 pm

thanks a lots….

Parveen Akther · May 15, 2019 at 1:18 pm

thank u sir ur my fav teacher

Ljk Productions · May 15, 2019 at 1:18 pm

Honors Bio class is impossible but this just made things a lot easier

Amit Kumar · May 15, 2019 at 1:18 pm

Early lysosome do not come in the path from Golgi to Lysosome.

Amit Kumar · May 15, 2019 at 1:18 pm

mannose 6 phosphate receptor is recycled..or not?

Amit Kumar · May 15, 2019 at 1:18 pm

why phosphorylation of mannose residues is done here?

Meditation Meditation · May 15, 2019 at 1:18 pm

Thank you for the good explanation!

Fa Mo · May 15, 2019 at 1:18 pm

thank you doctor .. you explain it sooo good ..

chandu regar · May 15, 2019 at 1:18 pm

so nice sir ji

Malti TANDAN · May 15, 2019 at 1:18 pm


Natรกssia Corrรชa · May 15, 2019 at 1:18 pm

Hi Shomu, I'm also a cellular and molecular biologist enthusiast and I was a Cellular Biology professor in Brazil. I always come to your videos to study and deepen in a topic. Thank you so much for your service. I have a question for you. Are you a professional, I mean, a regular classroom teacher on India? Thanks and keep up the great work! ๐Ÿ™‚

tuyelee das · May 15, 2019 at 1:18 pm

u r an awesome teacher..plz make a video of Lotka volterra model..

Aashish Kumar · May 15, 2019 at 1:18 pm

u r really awosem

MANGALDIP DE · May 15, 2019 at 1:18 pm


Alwina Anam · May 15, 2019 at 1:18 pm

thanks for the video ๐Ÿ˜

Ramchandra Suthar · May 15, 2019 at 1:18 pm

albert bruce

Ramchandra Suthar · May 15, 2019 at 1:18 pm

very nice analysis suman ji

Shalaka K.P · May 15, 2019 at 1:18 pm

Which reference is more than sufficient for CSIR …COOPER or ALBERT BRUCE .???

ุงู„ุชู…ูŠู…ูŠ ุงุจูˆ ู…ู‡ุฏูŠ · May 15, 2019 at 1:18 pm

plz add transelation arabic

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